Exploring indigenous Fungi for sustainable bioremediation of textile Dye
Acceptance: 16/07/2026
Published: 28/08/2026
Abstract
Textile dyes are considered as major environmental pollutants due to their complex chemical structures and resistance to natural degradation. In the present work fungal isolates were isolated and evaluated for biodegradation and decolourization of textile dyes for remediation purpose. Two fungal isolates Aspergillus niger and Aspergillus flavus were tested for their dye degradation efficiency against Remazol Brilliant Blue R (RBBR) and Methyl Violet dyes. Aspergillus niger showed better decolourisation efficiency of Remazol brilliant blue R(96.05%) and methyl violet (95.16%) than Aspergillus flavus. FTIR analysis confirmed the structural changes of the treated dye samples by shifting, disappearance and presence of peaks of the functional groups which indicated the biodegradation of the dye molecules. Enzyme analysis showed positive laccase and lignin peroxidase activity in both fungal isolates whereas manganese peroxidase activity was not significant. Phytotoxicity analysis using Vigna radiata seeds showed increased germination percentage after fungal treatment compared with untreated dye samples, indicating reduced toxicity. Statistical analysis viz. ANOVA, DMRT and paired t-test supported efficacy of the fungal treatment. The study indicates the potential of Aspergillus niger and Aspergillus flavus for eco-friendly and sustainable remediation of textile dyes.
Keynote: Mycoremediation Textile dye degradation, Biosorption, Laccase, FTIR, Phytotoxicity
Author Name:
Dr Preeti Mehta, Dr Deepali Rajwade, Dr Sadhana Jaiswal, Bharti Patle
Pages:
133-142
DOI Number:
10.5281/zenodo./22119376
Keyword:
Mycoremediation Textile dye degradation, Biosorption, Laccase, FTIR, Phytotoxicity
Reference
1. Alegbe, E. O., & Uthman, T. O. (2024). A review of history, properties, classification, applications and challenges of natural and synthetic dyes. Heliyon, 10(13).
2. Benkhaya, S., M’rabet, S., & El Harfi, A. (2020). A review on classifications, recent synthesis and applications of textile dyes. Inorganic Chemistry Communications, 115, 107891.
3. Ghaly, A. E., Ananthashankar, R., Alhattab, M., & Ramakrishnan, V. V. (2014). Production, characterization and treatment of textile effluents: A critical review. Journal of Chemical Engineering & Process Technology, 5(1), 182.
4. Kusumlata, Ambade, B., Kumar, A., & Gautam, S. (2024). Sustainable solutions: Reviewing the future of textile dye contaminant removal with emerging biological treatments. Limnological Review, 24(2), 126–149.
5. Lavanya, C., Dhankar, R., Chhikara, S., &Sheoran, S. (2014). Degradation of toxic dyes: A review. International Journal of Current Microbiology and Applied Sciences, 3(6), 189–199.
6. Lellis, B, Fávaro-Polonio, C. Z., Pamphile, J. A., & Polonio, J. C. (2019). Effects of textile dyes on health and the environment and bioremediation potential of living organisms. Biotechnology Research and Innovation, 3(2), 275–290
7. Dutta, S., Adhikary, S., Bhattacharya, S., Roy, D., Chatterjee, S., Chakraborty, A., … & Rajak, P. (2024). Contamination of textile dyes in aquatic environment: Adverse impacts on aquatic ecosystem and human health, and its management using bioremediation. Journal of Environmental Management, 353, 120103.
8. Nisa, F. U., Naseem, K., Aziz, A., Hassan, W., Fatima, N., Najeeb, J., … & Khan, M. E. (2025). Comparative analysis of dye degradation methods: unveiling the most effective and environmentally sustainable approaches, a critical review. Reviews in Inorganic Chemistry, 45(2), 275-306.
9. Sen, T. K., & Dawood, S. (2014). Review on dye removal from its aqueous solution into alternative cost effective and non-conventional adsorbents. Journal of Chemical and Process Engineering, 1, 1-7.
10. Lucas, M. S., Dias, A. A., Sampaio, A., Amaral, C., & Peres, J. A. (2007). Degradation of a textile reactive Azo dye by a combined chemical–biological process: Fenton’s reagent-yeast. Water research, 41(5), 1103-1109.
11. Ledakowicz, S., & Paździor, K. (2021). Recent achievements in dyes removal focused on advanced oxidation processes integrated with biological methods. Molecules, 26(4), 870.
12. Das, S., Cherwoo, L., & Singh, R. (2023). Decoding dye degradation: Microbial remediation of textile industry effluents. Biotechnology notes, 4, 64-76.
13. Jamal, M., Awadasseid, A., & Su, X. (2022). Exploring potential bacterial populations for enhanced anthraquinone dyes biodegradation: a critical review. Biotechnology Letters, 44(9), 1011–1025.
14.Nagra, K., Chaurasia, P. K., Bharati, S. L., Sharma, N., Kumar, J., & Sivalingam, A. M. (2025). Degradation of dyes by fungi: An overview on recent updates. The Microbe, 6, 100232.
15. Sevindik, M., Krupodorova, T., Gürgen, A., & Eraslan, E. C. (2026).Mycoremediation as a sustainable biotechnological approach for environmental pollution control. Environmental Sciences Europe, 38, 80.
16. Juárez-Hernández, J., Castillo-Hernández, D., Pérez-Parada, C., Nava-Galicia, S., Cuervo-Parra, J. A., Surian-Cruz, E., Díaz-Godínez, G., Sánchez, C., & Bibbins-Martínez, M. (2021). Isolation of fungi from a textile industry effluent and the screening of their potential to degrade industrial dyes. Journal of Fungi, 7(10), 805.
17. Sintakindi, A., &Ankamwar, B. (2021). Fungal biosorption as an alternative for the treatment of dyes in waste waters: A review. Environmental Technology Reviews, 10(1), 26–43.
18. Sen, S. K., Raut, S., & Raut, S. (2023). Mycoremediation of anthraquinone dyes from textile industries: A mini-review. BioTechnologia, 104(1), 85–91.
19.Ferreira, A. C. B., Tavares, Y. V., Fontana, N. R., Machado Pasin, T., Conte-Junior, C. A., & Contato, A. G. (2026). Bioremediation of synthetic dyes by white-rot fungi: Enzymatic mechanisms, biosorption, and environmental applications. Molecules, 31(7), 1085.
20. Kumar, C. G., Mongolla, P., Basha, A., Joseph, J., Sarma, V. U. M., & Kamal, A. (2011). Decolorization and biotransformation of triphenylmethane dye, methyl violet, by Aspergillus sp. isolated from Ladakh, India. Journal of Microbiology and Biotechnology, 21(3), 267–273.
21. Salem, S. S., Mohamed, A., El-Gamal, M., Talat, M., & Fouda, A. (2019). Biological decolorization and degradation of azo dyes from textile wastewater effluent by Aspergillus niger. Egyptian Journal of Chemistry, 62(10), 1799-1813.
22. Ghafoor, S., Ali, E., Rahim, F., Bukhari, D. A., Shafiq, S., Hussain, S. Z., & Rehman, A. (2024). Evaluation of azo dyes degradation potential of Aspergillus strains: A strategy for waste management. Journal of Hazardous Materials Advances, 16, 100475.
23. Ghanaim, A. M., El Mahdy, O. M., & Mohamed, H. I. (2025). Biodegradation of azo dyes by Aspergillus flavus and its bioremediation potential using seed germination efficiency. BMC Microbiology, 25(1), 7.
24. Ning, C., et al. (2018). Decolorization of a variety of dyes by Aspergillus flavus A5p1. Bioprocess and Biosystems Engineering, 41(4).
25. Chang, Y., Yang, D., Li, R., Wang, T., & Zhu, Y. (2021). Textile dye biodecolorization by manganese peroxidase: A review. Molecules, 26(15), 4403.
26. Singh, A. K., Bilal, M., Iqbal, H. M. N., & Raj, A. (2021). Lignin peroxidase in focus for catalytic elimination of contaminants: A critical review on recent progress and perspectives. International Journal of Biological Macromolecules 177, 58–82.
27. Jeyabalan, J., Veluchamy, A., Priyan, V. V., Kumar, A., Chandrasekar, R., & Narayanasamy, S. (2023). A review on the laccase assisted decolourization of dyes: Recent trends and research progress. Journal of the Taiwan Institute of Chemical Engineers, 151, 105081.
28. Singh, R. L., Singh, P. K., & Singh, R. P. (2015). Enzymatic decolorization and degradation of azo dyes–A review. International Biodeterioration & Biodegradation, 104, 21-31.
29.Mahmoud, M. S., Mostafa, M. K., Mohamed, S. A., Sobhy, N. A., & Nasr, M. (2017). Bioremediation of red azo dye from aqueous solutions by Aspergillus niger strain isolated from textile wastewater. Journal of environmental chemical engineering, 5(1), 547-554.
30. Asses, N., Ayed, L., Hkiri, N., & Hamdi, M. (2018). Congo red decolorization and detoxification by Aspergillus niger: removal mechanisms and dye degradation pathway. BioMed research international, 2018(1), 3049686.
31.Saratale, R. G., Saratale, G. D., Chang, J. S., &Govindwar, S. P. (2011). Bacterial decolorization and degradation of azo dyes: A review. Journal of the Taiwan Institute of Chemical Engineers, 42(1), 138–157.
32. Singh, S., Srivastava, V. C., & Mall, I. D. (2013). Mechanism of dye degradation during electrochemical treatment. The Journal of Physical Chemistry C, 117(29), 15229-15240.
33. Kumar, C. G., Mongolla, P., Basha, A., Joseph, J., Sarma, V. U. M., & Kamal, A. (2011). Decolorization and biotransformation of triphenylmethane dye, methyl violet, by Aspergillus sp. isolated from Ladakh, India. Journal of microbiology and biotechnology, 21(3), 267-273.
Writer Name
Dr Preeti Mehta, Dr Deepali Rajwade, Dr Sadhana Jaiswal, Bharti Patle
Pages
133-142
DOI Numbers
10.5281/zenodo./22119376
Our Social Networks
AD EDUXIAN Publication is dedicated to advancing educational research and providing innovative learning solutions. We offer a wide range of services including publishing, editing, design, and marketing to support authors and researchers in their academic and professional endeavors.
Never miss any important news. Subscribe to our newsletter.
